Guide Summary

This is an example of the procedure we might preform when brewing in order to help us have a quick reference sheet. It contains no explanations and just has day-of procedures.

• Clear work area
• Sweep and mop
• Hang plastic sheets
• Install and turn on HEPA air purifier
• Sanitize work surface
• Clean floor once more
• Begin wearing shoe covers and garbs in clean room
• Preheat oven to 200°C
• Wash, rinse, and wrap items for depyrogenation
• Put in oven once pre-heated, set timer for 2 hours
• Prepare autoclave with distilled water
• Wash, rinse, and wrap items for sterilization
• Autoclave for 1 hour.
• Wipe down everything else with IPA
• Setup laminar flow hood, leave on
• Remove items from oven and autoclave, stage in clean room
• Inside flow hood, mix in beaker:
  • ____mL MCT oil
  • ____mL Benzyl Alcohol
  • ____mL Benzyl Benzoate
  • ____mg Estradiol Enanthate
• Filter ____mL aside for sterility testing⁺
• Filter rest into vials, place stoppers immediately. Repeat.
• Use clean filter to do a second pass filtration into vials, cap⁺.
• Set 2nd pass filter aside for sterility testing⁺.
• Cap.
• Bubble point test
  • Listed bubble point: ____psi
  • Today’s air pressure: ____psi
  • Starting air volume: ____mL
  • Actual bubble point volume: ____mL
  • Actual bubble point (calculator): ____psi
• Place 2nd pass filter on agar for testing⁺.
• Make a record
• Label vials